Equipments
- Multispectral Confocal Leica TCS SP8 with a high speed module and a STED 3X module for super-resolution. Laser lines: 405 nm, 488 nm and white laser for 470-670 nm excitation. Depletion lines: 592, 660 nm and 775nm. Two hybrid detectors.
- Light microscope Leica DM5500B and DFC7000T digital color camera for the detection of light transmitted images, polarized light and fluorescence.
- Computer workstations for fluorescence and confocal processing and analysis images (LAS X, Fiji / ImageJ, Huygens).
- Auxiliary equipment: incubator implemented in the microscope with temperature and CO2 control, centrifuge, laminar flow chamber, freezer, CO2incubator.
Applications
Microscopy Techniques:
- Multichannel confocal and transmission imaging of living cells or fixed samples
- Three-dimensional analysis and relief maps
- Multidimensional in vivo time-lapse experiments: tracking, intracellular calcium, membrane potential, others
- High speed Confocal Microscopy
- Colocalization studies
- Quantitative fluorescence studies
- Techniques F: FRET, FRAP, photoactivation...
- Spectrum analysis of fluorescence
- Microscopy for multi-layered experiments and mosaics
- Transmitted light microscopy: BF, DIC and polarized light.
- Conventional fluorescence microscopy
- Image processing and quantification with specialized programs: LAS X, ImageJ, Fiji
- Nanoscopy imaging: HyVolution, STED 3X (Stimulated emission depletion 3D)
Sample Preparation:
- Sample preparation by transmitted light and fluorescence microscopy of different types of materials (biological and non-biological)
- Chemical bindings and cutting of biological materials
- Immunofluorescence in cells and tissues
- Methodologies for in vivo cell analysis and physiological studies: specific fluorochromes, ion markers (calcium), lectins and fluorescent proteins